Interferon-γ human I3265 from MilliporeSigma

Product Specs
ItemInterferon-γ human
CompanyMilliporeSigma
Price Pricing Info Supplier Page View Company Product Page
Catalog NumberI3265
Molecular Weight~17 kDa
Specieshuman
Molecule Namecow. , AGC1
NCBI Full Gene Nameaggrecan
NCBI Gene AliasesAGC1
Applicationsmammalian cell culture
FormatLyophilized from phosphate buffered saline
Quantity0.1MG, 0.1MG

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MilliporeSigma

400 Summit Drive

Burlington, Massachusetts 01803

United States

Phone: (800)-645-5476
(800)-645-5439

Company Profile

Website: www.SigmaAldrich.com/

(1) IL-4 and IL-13 induce equivalent expression of traditional M2 markers and modulation of reactive oxygen species in human macrophagesScientific ReportsNovember 10, 2023Tara E. Scott, Caitlin V. Lewis, Mingyu Zhu, Chao Wang, Chrishan S. Samuel, Grant R. Drummond, Barbara K. Kemp-HarperM-CSF and macrophages were either left untreated (MΦ), treated with 20 ng/ml IFN-γ (Sigma-Aldrich no. I3265) and 100 ng/ml lipopolysaccharide (LPS; Sigma-Aldrich no. L2630, E. coli 0111:B4 strain) for M1 polarisation,

(2) Differential Gene Expression in Activated Microglia Treated with Adenosine A2A Receptor Antagonists Highlights Olfactory Receptor 56 and T-Cell Activation GTPase-Activating Protein 1 as Potential Biomarkers of the Polarization of Activated MicrogliaCellsSeptember 5, 2023Alejandro Lillo, Joan Serrano-Marín, Jaume Lillo, Iu Raïch, Gemma Navarro, Rafael Francoobtained from PanReac AppliChem (Chicago, IL, USA), lipopolysaccharide (L4391-1MG) and human interferon-γ (I3265-1MG) were obtained from Sigma-Aldrich (St. Louis, MO, USA), 2-Cl-IB-MECA (1104), and SCH 58261 (2270)

(3) Endogenous IFITMs boost SARS-coronavirus 1 and 2 replication whereas overexpression inhibits infection by relocalizing ACE2iScienceApril 21, 2023Qinya Xie, Caterina Prelli Bozzo, Laura Eiben, Sabrina Noettger, Dorota Kmiec, Rayhane Nchioua, Daniela Niemeyer, Meta Volcic, Jung-Hyun Lee, Fabian Zech, et al.science,Cat# 11101-1 Recombinant IFN-β,R&D systems,Cat# 8499-IF-010 Recombinant IFN-γ,Sigma-Aldrich,Cat# I3265 Recombinant IFN- λ3,CHO,Cat# 5259-IL-025 Cytochalasin D,ThermoFisher,Cat# PHZ1063 Agarose,Sigma-Aldrich,Cat#

(4) A Whole-Genome Sequencing Study Implicates GRAMD1B in Multiple Sclerosis SusceptibilityGenes (Basel)December 16, 2022Federica Esposito, Ana Maria Osiceanu, Melissa Sorosina, Linda Ottoboni, Bryan Bollman, Silvia Santoro, Barbara Bettegazzi, Andrea Zauli, Ferdinando Clarelli, Elisabetta Mascia, et al.specificity of the antibody. 2.10. Reagents Stock solutions of TNFα, IFNγ, LPS (Sigma-Aldrich; T0157, I3265 and L2654, respectively), IFNβ (Rebif 44, Merck Serono, Darmstadt, Germany), or macrophage colony-stimulating

(5) Airway surface hyperviscosity and defective mucociliary transport by IL-17/TNF-α are corrected by beta-adrenergic stimulusJCI InsightNovember 22, 2022Daniela Guidone, Martina Buccirossi, Paolo Scudieri, Michele Genovese, Sergio Sarnataro, Rossella De Cegli, Federico Cresta, Vito Terlizzi, Gabrielle Planelles, Gilles Crambert, et al.Sigma-Aldrich), IL-4 (10 ng/mL, 14269, Sigma-Aldrich), IFN-α (10 ng/mL, H6041, Sigma-Aldrich), IFN-γ (10 ng/mL, I3265, Sigma-Aldrich), IL-1β (10 ng/mL H6291, Sigma-Aldrich), or IL-6 (10 ng/mL, I1395, Sigma-Aldrich). Immunofluorescence

(6) Evaluation of the Effects of Harmine on β-cell Function and Proliferation in Standardized Human Islets Using 3D High-Content Confocal Imaging and Automated AnalysisFront Endocrinol (Lausanne)July 4, 2022Alexandra C. Title, Maria Karsai, Joan Mir-Coll, Özlem Yavas Grining, Chantal Rufer, Sebastian Sonntag, Felix Forschler, Sayro Jawurek, Thomas Klein, Burcak Yesildagfor 4 days in parallel with cytokines: IL-1β (Sigma-Aldrich #I17001, 20 ng/mL), IFNγ (Sigma-Aldrich #I3265, 100 ng/mL), and TNFα (Sigma-Aldrich #PHC3016, 100 ng/mL). Luminescence was measured using a Tecan Spark

(7) Therapeutic Benefit in Rheumatoid Cachexia Illustrated Using a Novel Primary Human Triple Cell Coculture ModelInternational journal of inflammationJanuary 1, 2022Ollewagen T, Tarr GS, Myburgh KH, Reuter H, Smith C.GM-CSF, 50 ng/ml lipopolysaccharide (LPS; L2762, Sigma-Aldrich), and 20 ng/ml interferon-γ (IFN-γ; I3265, Sigma-Aldrich) for 24 hours. 2.6. Cell Phenotype Confirmation To confirm primary human myoblast (PHM)

(8) Semen microbiota and cytokines of healthy and infertile menAsian Journal of AndrologyJanuary 1, 2022AnastasiaV Bekpergenova, Natalya B Bukharin, NatalyaB Perunova, Irina N Chaynikova, Anastasia V Bekpergenova, Taisiya A Bondarenko, Michael D KuzminDarmstadt, Germany). The resulting supernatant was added to recombinant cytokines: interferon-γ (IFN-γ: I3265-1MG), tumor necrosis factor-α (TNF-α: T6674-10UG), interleukin-17 (IL-17: SRP3080), interleukin-1Ra

(9) Luciferase reporter assays to monitor interferon signaling modulation by SARS-CoV-2 proteinsSTAR protocolsDecember 17, 2021Hirschenberger M, Hayn M, Laliberté A, Koepke L, Kirchhoff F, Sparrer KMJ.(FBS),Gibco,Cat#10270106 Glycerol,Sigma-Aldrich,Cat#G5516 IFN-α2,Sigma-Aldrich,Cat#SRP4594 IFN-β,Merck,Cat#IF014 IFN-γ,Sigma-Aldrich,Cat#I3265 IFN-λ1,R&D Systems,Cat#1598-IL Immobilon-FL PVDF membrane,Merck Millipore,Cat#IPVH00010 KCl,Applichem,Cat#A2939 KH2PO4,Applichem,Cat#A1043 L-glutamine,PAN-Biotech,Cat#P04-80100 MES-SDS

(10) Cytotoxic T cells are able to efficiently eliminate cancer cells by additive cytotoxicityNature communicationsSeptember 1, 2021Weigelin B, den Boer AT, Wagena E, Broen K, Dolstra H, de Boer RJ, Figdor CG, Textor J, Friedl P.streptomycin (100 U/ml each; PAA, P11-010). Cells were stimulated with human IFNγ (200 U/ml; SIGMA, I3265) for 48 h prior to co-culture experiments. The identity of B16F10, MCF-7, MV3, and BLM cells was verified

(11) Transfer learning between preclinical models and human tumors identifies a conserved NK cell activation signature in anti-CTLA-4 responsive tumorsGenome medicineAugust 11, 2021Davis-Marcisak EF, Fitzgerald AA, Kessler MD, Danilova L, Jaffee EM, Zaidi N, Weiner LM, Fertig EJ.preclinical repository), 50 ng/mL IL-18 (Invitrogen, cat#rcyec-hil18), or 500 U/mL IFNg (Sigma-Aldrich, cat# I3265) for 24 h. Cell pellets were collected and processed for rt-qPCR as described above. Cell lines or expanded

(12) Systematic functional analysis of SARS-CoV-2 proteins uncovers viral innate immune antagonists and remaining vulnerabilitiesCell reportsMay 18, 2021Hayn M, Hirschenberger M, Koepke L, Nchioua R, Straub JH, Klute S, Hunszinger V, Zech F, Prelli Bozzo C, Aftab W, et al.(FBS),GIBCO,Cat#10270106 Glycerol,Sigma-Aldrich,Cat#G5516 HCl,VWR,Cat#20252 HEPES,Sigma-Aldrich,Cat#H3375 IFN-α2,Sigma-Aldrich,Cat#SRP4594 IFN-β,Merck,Cat#IF014 IFN-γ,Sigma-Aldrich,Cat#I3265 IFN-λ1,R&D Systems,Cat#1598-IL IL-1α,R&D Systems,Cat#200-LA Immobilon-FL PVDF membrane,Merck Millipore,Cat#IPVH00010 iST-kit,Preomics,Cat#P.O.00027 L-glutamine,PAN-Biotech,Cat#P04-80100 LEGENDplex

(13) Higher Prevalence of Bacteroides fragilis in Crohn's Disease Exacerbations and Strain-Dependent Increase of Epithelial ResistanceFrontiers in microbiologyJanuary 1, 2021Becker HEF, Jamin C, Bervoets L, Boleij A, Xu P, Pierik MJ, Stassen FRM, Savelkoul PHM, Penders J, Jonkers DMAE.factor α (TNF-α; Sigma, ref.: T6674) in combination with 100 ng/ml interferon γ (IFN-γ; Sigma, ref.: I3265) for 1 h, before B. fragilis supernatant was added luminally. After another 24 h of co-incubation, TEER

(14) Targeted pharmacological therapy restores β-cell function for diabetes remissionNature MetabolismFebruary 1, 2020Stephan Sachs et al.(TNFα, 10 ng ml−1, Thermo Fisher Scientific PHC3016), interferon gamma (IFNγ, 10 ng ml−1, SigmaAldrich I3265) and interleukin-1beta (IL-1β, 2 ng ml−1, Sigma-Aldrich I17001), was prepared in PBS containing 0.1%

(15) Distinct Redox Signalling following Macrophage Activation Influences Profibrotic ActivityJournal of Immunology ResearchNovember 11, 2019Caitlin V. Lewis, Antony Vinh, Henry Diep, Chrishan S. Samuel, Grant R. Drummond, Barbara K. Kemp-Harperleft untreated (MΦ) or treated with either a combination of 5 ng/ml interferon-γ (IFN-γ; Sigma-Aldrich no. I3265) and 10 ng/ml lipopolysaccharide (LPS; Sigma-Aldrich no. L2630, E. coli 0111:B4 strain) or with 25 ng/ml

(16) IFI16 Targets the Transcription Factor Sp1 to Suppress HIV-1 Transcription and Latency ReactivationCell host & microbeJune 12, 2019Hotter D, Bosso M, Jønsson KL, Krapp C, Stürzel CM, Das A, Littwitz-Salomon E, Berkhout B, Russ A, Wittmann S, et al.07851 Human Interferon α 2a,pbl assay science,Cat# 11101-1 Human Interferon γ,Sigma-Aldrich,Cat# I3265 Dynabeads Human T-Activator CD3/CD28,ThermoFisher,Cat# 111.32D Tumor Necrosis Factor-α human,Sigma-Aldrich,Cat#

(17) Intraperitoneal cancer-immune microenvironment promotes peritoneal dissemination of gastric cancerOncoimmunologyJanuary 1, 2019Sakamoto S, Kagawa S, Kuwada K, Ito A, Kajioka H, Kakiuchi Y, Watanabe M, Kagawa T, Yoshida R, Kikuchi S, et al.were primed for 24 hours with fresh medium supplemented with interferon-γ (20 ng/ml; Sigma-Aldrich, #I3265) + c (1 μg/ml; Sigma-Aldrich) to polarize into the M1 phenotype and with IL-4 (20 ng/ml; Sigma-Aldrich,

(18) Suppression of T cells by mesenchymal and cardiac progenitor cells is partly mediated via extracellular vesiclesHeliyonJune 6, 2018F. van den Akker, K.R. Vrijsen, J.C. Deddens, J.W. Buikema, M. Mokry, L.W. van Laake, P.A. Doevendans, J.P.G. Sluijterper well in a 48-wells plate. In case of the pre-conditioning experiments, 20 ng/mL IFN-gamma (Sigma I3265) was added. After 24 hours, the medium was removed and 5.0*104 freshly isolated, CFSE-labeled T cells

(19) A Direct Droplet Digital PCR Method for E. coli Host Residual DNA QuantificationPharmacology & PharmacyJanuary 1, 2018Jeremy Anderson, Musaddeq HussainRP-IR) and three protein molecules, human interferon-α A (IFN-α, Cat# I4276), interferon-γ (IFN-γ, Cat# I3265) and insulin like growth factor (IGF, Cat# I3769) purchased from Sigma; all were manufactured in E. coli

(20) Histone chaperone HIRA deposits histone H3.3 onto foreign viral DNA and contributes to anti-viral intrinsic immunityNucleic Acids ResearchNovember 16, 2017Taranjit Singh Rai, Mandy Glass, John J. Cole, Mohammad I. Rather, Morgan Marsden, Matthew Neilson, Claire Brock, Ian R. Humphreys, Roger D. Everett, Peter D. Adamsappropriate. Recombinant IFNs were bought from PBL (Human IFN-β -11410–2) and Sigma (IFN-α-SRP4596 and IFN-γ-I3265). ELISA (PBL Assay Science 41410) was performed according to the manufacturer’s instructions. Viruses HCMV

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Interferon-γ human from MilliporeSigma

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Interferon-γ human from MilliporeSigma

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